| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on August 28, 2001
Branch of the Novartis Research Foundation, Friedrich Miescher Institute for Biomedical Research, Basel CH-4058
Corresponding Author: hofsteenge{at}fmi.ch
The final chemical structure of a newly synthesized protein is often only attained after further covalent modification. Ideally, a comprehensive proteome analysis includes this aspect, a task that is complicated by our incomplete knowledge of the range of possible modifications, and often by the lack of suitable analysis methods. Here we present two recently discovered, unusual forms of protein glycosylation, i.e. C-mannosylation and O-fucosylation. Their analysis by a combined mass spectrometric approach is illustrated with peptides from the thrombospondin type 1 repeats (TSRs) of the axonal guidance protein rF-spondin. Nano-ESI-MSMS of isolated peptides showed that 8 out of 10 Trp residues in the TSRs of F-spondin are C-mannosylated. O-fucosylation sites were determined by a recently established nano-ESI Q-TOF MSMS approach. Four out of 5 TSRs carry the disaccharide Hex-dHex-O-Ser/Thr in close proximity to the C-mannosylation sites. In analogy to thrombospondin-1, we assume this to be Glc-Fuc-O-Ser/Thr. Our current knowledge of these glycosylations will be discussed.
Revised on September 17, 2001
Accepted on September 19, 2001
C-mannosylation and O-fucosylation of thrombospondin type 1 repeats
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Zhou, L. W. Li, Q. Yan, B. Petryniak, Y. Man, C. Su, J. Shim, S. Chervin, and J. B. Lowe Notch-dependent control of myelopoiesis is regulated by fucosylation Blood, July 15, 2008; 112(2): 308 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Gebauer, S. Muller, F.-G. Hanisch, M. Paulsson, and R. Wagener O-Glucosylation and O-Fucosylation Occur Together in Close Proximity on the First Epidermal Growth Factor Repeat of AMACO (VWA2 Protein) J. Biol. Chem., June 27, 2008; 283(26): 17846 - 17854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hess, J. J. Keusch, S. A. L. Oberstein, R. C. M. Hennekam, and J. Hofsteenge Peters Plus Syndrome Is a New Congenital Disorder of Glycosylation and Involves Defective O-Glycosylation of Thrombospondin Type 1 Repeats J. Biol. Chem., March 21, 2008; 283(12): 7354 - 7360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Muroi, S. Manabe, M. Ikezaki, Y. Urata, S. Sato, T. Kondo, Y. Ito, and Y. Ihara C-Mannosylated peptides derived from the thrombospondin type 1 repeat enhance lipopolysaccharide-induced signaling in macrophage-like RAW264.7 cells Glycobiology, September 1, 2007; 17(9): 1015 - 1028. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Manya, T. Suzuki, K. Akasaka-Manya, H.-K. Ishida, M. Mizuno, Y. Suzuki, T. Inazu, N. Dohmae, and T. Endo Regulation of Mammalian Protein O-Mannosylation: PREFERENTIAL AMINO ACID SEQUENCE FOR O-MANNOSE MODIFICATION J. Biol. Chem., July 13, 2007; 282(28): 20200 - 20206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shi, C. Ge, Y. Luo, X. Hou, R. S. Haltiwanger, and P. Stanley The Threonine That Carries Fucose, but Not Fucose, Is Required for Cripto to Facilitate Nodal Signaling J. Biol. Chem., July 13, 2007; 282(28): 20133 - 20141. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Ricketts, M. Dlugosz, K. B. Luther, R. S. Haltiwanger, and E. M. Majerus O-Fucosylation Is Required for ADAMTS13 Secretion J. Biol. Chem., June 8, 2007; 282(23): 17014 - 17023. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. W. Wang, M. Dlugosz, R. P. T. Somerville, M. Raed, R. S. Haltiwanger, and S. S. Apte O-Fucosylation of Thrombospondin Type 1 Repeats in ADAMTS-like-1/Punctin-1 Regulates Secretion: IMPLICATIONS FOR THE ADAMTS SUPERFAMILY J. Biol. Chem., June 8, 2007; 282(23): 17024 - 17031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kozma, J. J. Keusch, B. Hegemann, K. B. Luther, D. Klein, D. Hess, R. S. Haltiwanger, and J. Hofsteenge Identification and Characterization of abeta1,3-Glucosyltransferase That Synthesizes the Glc-beta1,3-Fuc Disaccharide on Thrombospondin Type 1 Repeats J. Biol. Chem., December 1, 2006; 281(48): 36742 - 36751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sato, M. Sato, K. Kiyohara, M. Sogabe, T. Shikanai, N. Kikuchi, A. Togayachi, H. Ishida, H. Ito, A. Kameyama, et al. Molecular cloning and characterization of a novel human {beta}1,3-glucosyltransferase, which is localized at the endoplasmic reticulum and glucosylates O-linked fucosylglycan on thrombospondin type 1 repeat domain Glycobiology, December 1, 2006; 16(12): 1194 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Loriol, F. Dupuy, R. Rampal, M.A. Dlugosz, R.S. Haltiwanger, A. Maftah, and A. Germot Molecular evolution of protein O-fucosyltransferase genes and splice variants Glycobiology, August 1, 2006; 16(8): 736 - 747. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Buskas, S. Ingale, and G.-J. Boons Glycopeptides as versatile tools for glycobiology Glycobiology, August 1, 2006; 16(8): 113R - 136R. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tossavainen, T. Pihlajamaa, T. K. Huttunen, E. Raulo, H. Rauvala, P. Permi, and I. Kilpelainen The layered fold of the TSR domain of P. falciparum TRAP contains a heparin binding site. Protein Sci., July 1, 2006; 15(7): 1760 - 1768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Luo, A. Nita-Lazar, and R. S. Haltiwanger Two Distinct Pathways for O-Fucosylation of Epidermal Growth Factor-like or Thrombospondin Type 1 Repeats J. Biol. Chem., April 7, 2006; 281(14): 9385 - 9392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Luo, K. Koles, W. Vorndam, R. S. Haltiwanger, and V. M. Panin Protein O-Fucosyltransferase 2 Adds O-Fucose to Thrombospondin Type 1 Repeats J. Biol. Chem., April 7, 2006; 281(14): 9393 - 9399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Marteau, N. S. Gonzalez, D. Communi, M. Goldman, J.-M. Boeynaems, and D. Communi Thrombospondin-1 and indoleamine 2,3-dioxygenase are major targets of extracellular ATP in human dendritic cells Blood, December 1, 2005; 106(12): 3860 - 3866. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Li, L. Cui, D. L. Rock, and J. Li Novel Glycosidic Linkage in Aedes aegypti Chorion Peroxidase: N-MANNOSYL TRYPTOPHAN J. Biol. Chem., November 18, 2005; 280(46): 38513 - 38521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ihara, S. Manabe, M. Kanda, H. Kawano, T. Nakayama, I. Sekine, T. Kondo, and Y. Ito Increased expression of protein C-mannosylation in the aortic vessels of diabetic Zucker rats Glycobiology, April 1, 2005; 15(4): 383 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Luo and R. S. Haltiwanger O-Fucosylation of Notch Occurs in the Endoplasmic Reticulum J. Biol. Chem., March 25, 2005; 280(12): 11289 - 11294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Ervin, L. E. Ball, R. K. Crouch, and K. L. Schey Phosphorylation and Glycosylation of Bovine Lens MP20 Invest. Ophthalmol. Vis. Sci., February 1, 2005; 46(2): 627 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. V. Narizhneva, V. J. Byers-Ward, M. J. Quinn, F. J. Zidar, E. F. Plow, E. J. Topol, and T. V. Byzova Molecular and Functional Differences Induced in Thrombospondin-1 by the Single Nucleotide Polymorphism Associated with the Risk of Premature, Familial Myocardial Infarction J. Biol. Chem., May 14, 2004; 279(20): 21651 - 21657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Sturla, R. Rampal, R. S. Haltiwanger, F. Fruscione, A. Etzioni, and M. Tonetti Differential Terminal Fucosylation of N-Linked Glycans Versus Protein O-Fucosylation in Leukocyte Adhesion Deficiency Type II (CDG IIc) J. Biol. Chem., July 11, 2003; 278(29): 26727 - 26733. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Journal of Biological Chemistry |
| Journal of Lipid Research | ASBMB Today |
